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This facilitates their potential future development and efficient application for sustainable agriculture.

Our study promotes the understanding of the insecticidal mechanism of Vip3 proteins and illustrates an easily implementable strategy to increase the insecticidal potency of Vip3Aa's. GraphPad Prism edit GraphPad Prism is a commercial scientific 2D graphing and statistics software for Windows and Mac OS desktop computers. Software features include nonlinear regression, with functionalities including the removal of outliers, comparisons of models, comparisons of curves, and interpolation of standard curves. GraphPad Prism Tutorial 2 - Making XY Graphs protocolplace 10.8K subscribers Subscribe 1.9K Share 136K views 4 years ago This is the second video in our series dedicated to the powerful data. We further demonstrated that by adding more cleavage sites between DI and DII of Vip3Aa, its proteolysis efficiency by midgut proteases can be significantly increased, and thus enhance its insecticidal activity against Spodoptera frugiperda and Helicoverpa armigera larvae. GraphPad Prism is a commercial scientific 2D graphing and statistics software for Windows and Mac OS desktop computers.

In this study, we found that in addition to the preliminary digestion sites lysine, there are multiple cleavage activation sites in the linker region between domain I (DI) and DII of Vip3Aa. They are considered to be a promising toxic candidate for the management of various detrimental pests. Vip3 family proteins from Bacillus thuringiensis have distinct characteristics from known insecticidal Cry toxins and show efficient insecticidal activity against several detrimental lepidopteran pests. Microbially derived protein-based biopesticides have become a vital element in pest management strategies.